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4 edition of Autoradiography of diffusible substances found in the catalog.

Autoradiography of diffusible substances

International Conference on High Resolution Autoradiography of Diffusible Substances (1968 University of Chicago)

Autoradiography of diffusible substances

by International Conference on High Resolution Autoradiography of Diffusible Substances (1968 University of Chicago)

  • 166 Want to read
  • 20 Currently reading

Published by Academic P in New York, London .
Written in English

    Subjects:
  • Cytology -- Technique -- Congresses.,
  • Histochemistry -- Technique -- Congresses.,
  • Autoradiography -- Congresses.

  • Edition Notes

    "Based on a series of lectures presented at an International Conference on High Resolution Autoradiography of Diffusible Substances held at the University of Chicago, June 2-4 1968, sponsored by the Pharmaceutical Manufacturers Association Foundation, Inc., Washington D.C.".

    Statementedited by Lloyd J. Roth and Walter E. Stumpf.
    ContributionsRoth, Lloyd Joseph., Stumpf, Walter E.
    The Physical Object
    Paginationxix,371p. :
    Number of Pages371
    ID Numbers
    Open LibraryOL14939211M
    ISBN 100125985509

      Freeze-fracture autoradiography (FFA) was introduced in as a new method for electron microscopic autoradiography of diffusible compounds (Fisher and Branton, Rix et al.). With the original technique, the film monolayer was applied to . Radioactive substances are introduced into the test material either in a given chemical form or tagged with certain metabolic precursors. Nucleic acid can be made radioactive by incorporation of radioactive phosphate during nucleic acid synthesis. The following three types of radiations are used in autoradiography.

      There are basically two methods of working with tissue containing diffusible substances. One is to Freeze-dry the tissue (i.e., sublimate it) and the other is to work with frozen sections directly. L. J., eds., in The Autoradiography of Diffusible Substances, Academic Press, N.Y., (), which gives an excellent and useful introduction. Localization of drugs and other diffusible compounds with dry-mount and thaw-mount autoradiography. For the in vivo cellular and subcellular localization of non-covalently bound drugs (diffusible compounds) no method existed. Development of a suitable histochemical method was considered very difficult (C.P. Leblond), even impossible (H. Levi).

    This book, as its title indicates, consists of three chapters describing in considerable detail the techniques of autoradiography for both the light and the electron microscope, and six chapters dealing with the application of autoradiography to such problems as DNA . References.- 12 Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistry.- 1 Introduction.- 2 Cryoultramicrotomy for the Autoradiography of Diffusible Substances.- 3 Thin Cryosections in Histochemistry.- 4 Conclusions.- References.- 13 Scanning Electron Microscopy and X-Ray Microanalysis of Frozen-Hydrated Bulk Samples.- 1 Introduction.


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Autoradiography of diffusible substances by International Conference on High Resolution Autoradiography of Diffusible Substances (1968 University of Chicago) Download PDF EPUB FB2

"Based on a series of lectures presented at an International Conference on High Resolution Autoradiography of Diffusible Substances held at the University of Chicago, June" Description: xix, pages illustrations 24 cm.

Macroautoradiography 4. Light Microscopic Autoradiography-Fixable Substances 5. Light Microscopic Autoradiography–Diffusible Substances 6. Autoradiography of Receptors 7.

Electron Microscopic Autoradiography–Preparative Methods 8. Efficiency 9. Resolution Quantification of EM Autoradiographs Autoradiography in Relation to Other Pages:   Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro- stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography.

Practical and theoretical details of the technique, together Book Edition: 1. Autoradiography of diffusible substances: localization of steroids in the testis Publication Publication. The testis contains two cell compartments respectively having an endocrine and an exocrine function.

The seminiferous tubules contain a cell renewal system concerned with the production of spermatozoa, and are the exocrine part of the : P.M. Frederik. Review of cryotechniques for autoradiography of diffusible substances Use of cryosections Use of frozen dried tissue Discussion of own experimental work Introduction Discussion of the freeze dry technique Summary and conclusions Author: P.M.

Frederik. Autoradiography of diffusible substances: localization of steroids in the testis: Author: P.M. Frederik: Degree grantor: Erasmus MC: University Medical Center Rotterdam: The incorporated radioactivity was subsequently localized in tissue sections by autoradiography both at the level of the light and of the electron microscope.

Before. Light Microscopic Autoradiography --Fixable Substances Autoradiography of diffusible substances book Microscopic Autoradiography --Diffusible Substances Autoradiography of Receptors Electron Microscopic Autoradiography --Preparative Methods Efficiency Resolution Quantification of EM Autoradiographs Autoradiography in Relation to Other Techniques.

Rent or Buy Autoradiography - by Baker, John for as low as $ at Voted #1 site for Buying Textbooks. AUTORADIOGRAPHY OF DIFFUSIBLE COMPOUNDS Cryopump (Thermovac Industries, Copiague, L.I., New York), which is kept within the cryostat.

The specimen chamber is attached to the Cryopump with the 0-ring joint seating itself as a. Autoradiography of diffusible substances: localization of steroids in the testis. By P.M. Frederik. Download PDF (37 KB) Abstract. The testis contains two cell compartments respectively having an endocrine and an exocrine function.

The seminiferous tubules contain a cell renewal system concerned with the production of spermatozoa, and are the. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies.

Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro- stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography.

Practical and theoretical details of the technique, together with modifications made, are covered in each chapter. Autoradiography of diffusible substances: verification of the specificity of the localization by correlated physiological, biochemical, and pharmacological studies.

Mills JW. Recent advances in autoradiographic technique make it possible to localize quite a diverse class of soluble and/or diffusible substances.

Autoradiography permits the localization of radioactive substances in tissues by means of emitted radiation effects on photographic emulsions. Silver bromide crystals present in the emulsion act as microdetectors of radioactivity in the same way that they respond to light in common photography. • Autoradiography, although used to locate the radioactive substances, it can also be used for quantitative estimation by using densitometer.

• The first autoradiography was obtained accidently around when a blackening was produced on emulsions of silver chloride and iodide by uranium salts observed by Niepce de St. Victor. A method is described for preparing whole body sections of rats, 7 days old, which had been injected with Mo and Se.

Autoradiography of diffusible substances--a practical approach. (PMID) Abstract Citations; Related Articles; Data; BioEntities; External Links ' ' Ison EJ, ' ' Sheridan PJ The American Journal of Medical Technology [01 Jan47(1)] Type.

Appleton TC () The possibilities of locating soluble labelled compounds by electron microscope autoradiography. In: Roth LJ, Stumpf WE (eds) Autoradiography of diffusible substances. Academic Press, London New York, pp – Google Scholar. Abstract.

In H ilde L evi wrote that in almost no analytical method was the term “quantitative” so misused as in autoradiography. If we take the term “quantitative” to mean that absolute amounts of a substance are measured, this would seem to be true. For the successful application of autoradiography for the localization of diffusible substances, not chemically bound to stable tissue constituents, special prerequisites must be considered.

Autoradiography 1. AUTORADIOGRAPHY: A BIO-ANALYTICAL TECHNIQUE BY RAHUL GAUR 2. INTRODUCTION Autoradiography is a bio-analytical technique used to visualize the radioactively labelled substances or molecules or fragments of molecules. It uses X-ray or photographic film to detect relative positions and intensities of radiolabeled bands in a gel or blot.book.

Autoradiography of Diffusible Substances. Autoradiography of Progesterone and Model Compound Entry and Distribution in Xenopus Laevis Oocytes (Progress in .On the removal of diffusible substances from the circulating blood of living animals by dialysis. Offprint from J. Pharm. & Exp. Therapeutics 5 8vo.

pp. Text illustrations. x mm. Original printed wrappers, slightly chipped at bottom edge. Very good copy. Book Id: .